COLMARm 13C-1H HSQC, HSQC-TOCSY and TOCSY Query and Verification

New version, see changing log here. You can still visit the old one here.
Visit a step by step how-to with figures here

STEP1, spectrum processing and peak picking:

Your name and institute*
Required HSQC spectrum file
Optional TOCSY spectrum file
Optional HSQC-TOCSY spectrum file
Optional user chosen session name
(Notice: spectra files can be nmrpipe (.ft2), sparky (.ucsf) or Topspin ASCII format (.txt)). The server use file extension to distinguish file format!

STEP2, referencing
Automatic by pattern matchings
DSS Alanine Leucine Glucose Lactic Acid
When doing auto-referencing, matching at least
out of known peaks.
1H: 13C:

STEP3, database query and match:
1H chemical shift cutoff (ppm): 13C chemical shift cutoff (ppm): ,  Matching ratio cutoff  

data for database query and display.

Start over
peak picking, referencing and database query
Load previous session:
You previous session id

(Type "example" into session id box to load in model mixture example.)


HSQC Lowest contour level: = 0

Control: Brush an area to Zoom in, or remove peaks within this area. , ,
Visualization:

Peak list:



Visualization options:

  • Zoom scale:
  • plot size:
  • Contour color: Gray Colorful.
  • Draw expected cross peaks from database experimental hybrid peaks or does not draw.
Show detail of one matched spin-system:
Data base peak Matched experimental peak Expected cross peaks
HSQC Lowest contour level: = 0

TOCSY Lowest contour level: = 0

HSQC-TOCSY Lowest contour level: = 0

Save peak and compound information to local file
Using Space or Comma as delimiter.

List of compounds in our database

Reference

Question or comment? Please contact us

Acknowledgement

This web server and the research that led to it is supported by the National Institutes of Health (Grant R01 GM 066041) and SECIM (Southeast Center for Integrated Metabolomics) grant U24 DK097209-01A1

*These information are only used for the compilation of our webserver usage data.

This server is last updated at April 27 2017 13:18:13.